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The end of Part 1 saw the unexpected arrival of Michaela Stirling (Masali Baduza), the cousin of John Stirling (Victor Alli), whom we met at the end of Season 3. (This character is the gender-flipped version of Michael Stirling from the books.) Over the second part of Season 4, the show establishes the lived-in friendship between Francesca, John, and Michaela, whose connection in Quinn's books is one of inseparable Three Musketeers energy, and sets up the hesitant, unspoken draw between Francesca and Michaela.
It is difficult to know exactly when the shift from gelatine to agar occurred. As often happens for scientific breakthroughs, agar was likely adopted incrementally alongside the use of other growth media. In 1913, for example, the first diagnosis of Serratia marcescens as a human pathogen was made by growing it on agar as well as on potatoes.